Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

DNA Damage Can Stall the Cell Cycle

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

Valence Bond Theory

Valence Bond Theory

Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SHY2 as a node in the regulation of root meristem development by auxin, brassinosteroids, and cytokinin.

Journal of integrative plant biology·2020

Same author

Dynamic tailoring of an optical skyrmion lattice in surface plasmon polaritons.

Optics express·2020

Same author

High-Efficiency, Broadband, Near Diffraction-Limited, Dielectric Metalens in Ultraviolet Spectrum.

Nanomaterials (Basel, Switzerland)·2020

Same author

Experimental Examination of Electrical Characteristics for Portland Cement Mortar Frost Damage Evaluation.

Materials (Basel, Switzerland)·2020

Same author

Synthesis of Imidazole-Based Medicinal Molecules Utilizing the van Leusen Imidazole Synthesis.

Pharmaceuticals (Basel, Switzerland)·2020

Same author

Mutagenesis for Improvement of Activity and Stability of Prolyl Aminopeptidase from Aspergillus oryzae.

Applied biochemistry and biotechnology·2020

Search research articles

Related Experiment Video

Updated: May 12, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

A Pt(II)-Dip complex stabilizes parallel c-myc G-quadruplex.

Jintao Wang¹, Kaihui Lu, Shuguang Xuan

¹Division of Chemistry and Biological Chemistry, Nanyang Technological University, 21 Nanyang Link, 637371, Singapore.

Chemical Communications (Cambridge, England)

|April 16, 2013

Summary

This summary is machine-generated.

A novel platinum complex, [Pt(Dip)2](PF6)2, effectively stabilizes G-quadruplex DNA structures. This compound shows high selectivity for parallel G-quadruplexes over duplex DNA, offering a promising tool for G-quadruplex research.

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Related Experiment Videos

Last Updated: May 12, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Area of Science:

Medicinal Chemistry
Bioinorganic Chemistry
Molecular Biology

Background:

G-quadruplexes (GQs) are non-canonical DNA secondary structures implicated in various cellular processes.
Targeting G-quadruplexes offers potential therapeutic strategies for diseases like cancer.
Development of selective G-quadruplex ligands is crucial for minimizing off-target effects.

Purpose of the Study:

To synthesize and characterize a new platinum-based G-quadruplex stabilizer.
To evaluate the binding affinity and selectivity of the novel compound towards G-quadruplex DNA.
To investigate the potential of aromatic anchors in enhancing G-quadruplex binding specificity.

Main Methods:

Microwave irradiation synthesis of the platinum complex [Pt(Dip)2](PF6)2.
Spectroscopic and analytical characterization of the synthesized complex.
DNA-binding studies to assess interactions with G-quadruplex and duplex DNA.

Main Results:

The platinum complex [Pt(Dip)2](PF6)2 was successfully synthesized using microwave irradiation.
The complex demonstrated high stabilization of G-quadruplex structures.
Negligible interaction was observed with duplex DNA, indicating high selectivity.
Aromatic anchors on the ligands conferred a strong binding preference for parallel G-quadruplexes.

Conclusions:

The novel platinum complex [Pt(Dip)2](PF6)2 is an effective and selective stabilizer of G-quadruplex DNA.
The compound's design, featuring aromatic anchors, promotes preferential binding to parallel G-quadruplexes.
This G-quadruplex stabilizer holds promise for further investigation in therapeutic applications and as a molecular probe.