Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Multi-species Conserved Sequences

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

How can biological databases support the new UN mechanism for benefit-sharing from digital sequence information?

Scientific data·2026

Same author

Multi-omic analysis of deep learning-derived phenotypes links ophthalmic imaging to cardiovascular and neurological traits.

Nature cardiovascular research·2026

Same author

A panel of medaka isogenic lines suggests individual, seasonal, and sexual genetic variation of <i>bdnf</i> gene expression in the brain.

Frontiers in behavioral neuroscience·2026

Same author

The Human Pleiotropic Map of GWAS Associations and Therapeutic Implications.

bioRxiv : the preprint server for biology·2026

Same author

AI in biocuration: challenges, opportunities, and a roadmap for sustainable integration.

Bioinformatics advances·2026

Same author

Medaka: a novel model for analyzing genome-environment interactions.

Trends in genetics : TIG·2026

Search research articles

Related Experiment Video

Updated: May 12, 2026

Microbial Communities in Nature and Laboratory - Interview

Microbial Communities in Nature and Laboratory - Interview

Published on: May 28, 2007

The future of DNA sequence archiving.

Guy Cochrane¹, Charles E Cook, Ewan Birney

¹EMBL-Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SD, United Kingdom. cochrane@ebi.ac.uk.

|April 17, 2013

Summary

This summary is machine-generated.

The International Nucleotide Sequence Database Collaboration needs to manage increasing DNA sequence data. A new graded system will use experiment reproducibility and sample availability to determine data compression levels for archived sequences.

More Related Videos

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Analyzing and Building Nucleic Acid Structures with 3DNA

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Related Experiment Videos

Last Updated: May 12, 2026

Microbial Communities in Nature and Laboratory - Interview

Microbial Communities in Nature and Laboratory - Interview

Published on: May 28, 2007

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Analyzing and Building Nucleic Acid Structures with 3DNA

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Area of Science:

Bioinformatics
Genomics Data Archiving

Background:

Current archiving practices treat all DNA sequences equally.
Rapidly increasing global sequence production necessitates new data management strategies.

Purpose of the Study:

To propose a differentiated treatment system for archiving DNA sequences.
To address the challenges posed by the exponential growth of genomic data.

Main Methods:

Implementing a graded system for data compression.
Assessing the ease of experimental reproduction for sequencing data.
Evaluating the relative availability of biological samples for resequencing.

Main Results:

A proposed system to apply lossy compression based on data characteristics.
Potential for efficient management of large-scale genomic archives.

Conclusions:

The proposed graded system offers a scalable solution for archiving vast amounts of DNA sequence data.
Differentiated data treatment is crucial for the future sustainability of nucleotide sequence archives.