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Updated: May 12, 2026

Efficient Techniques for Comprehensive Sampling of Accessible Tissues in Adult Xenopus
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Efficient Techniques for Comprehensive Sampling of Accessible Tissues in Adult Xenopus

Published on: June 10, 2025

Tissue sampling methods and standards for vertebrate genomics.

Pamela By Wong1, Edward O Wiley, Warren E Johnson

  • 1Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St,, Toronto, Ontario, M5S 3B2, Canada. bob.murphy@utoronto.ca.

Gigascience
|April 17, 2013
PubMed
Summary
This summary is machine-generated.

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This study outlines a standardized guide for collecting and storing tissue samples for large-scale genomic projects like the Genome 10K Project. Implementing these tissue acquisition standards ensures high-quality DNA and RNA for genetic sequence analysis.

Area of Science:

  • Genomics
  • Molecular Biology
  • Biobanking

Background:

  • Advancements in sequencing technologies enable high-throughput analysis of vertebrate genomes.
  • High-quality DNA and RNA acquisition and cell line establishment are crucial for genomic initiatives.
  • Standardized protocols are needed for consistent sample collection and storage.

Purpose of the Study:

  • To provide a legal and methodological guide for tissue acquisition and storage for the Genome 10K Project and similar genomic studies.
  • To establish a tiered system (one- to four-star) for assessing sample quality based on DNA/RNA quantity and preservation method.
  • To promote standardized practices in genomic data generation and biobanking.

Main Methods:

  • Development of a four-star rating system for tissue samples: four-star (banked tissue/cell cultures, RNA from multiple tissues, 1 mg DNA from a single individual), three-star (RNA, 1 mg DNA), two-star (700 μg DNA), and one-star (ethanol-preserved tissue).

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Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
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Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models

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Last Updated: May 12, 2026

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Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models

Published on: March 6, 2018

  • Consideration of species' natural history, institutional, and legal requirements for tissue collection.
  • Emphasis on detailed documentation of sample provenance for representative sampling.

    • Main Results:

    • A clear, tiered methodology for evaluating and standardizing tissue sample quality for genomic research.
    • Guidelines that accommodate varying levels of sample quality and preservation methods.
    • A framework to ensure consistency and reliability in samples used for high-throughput sequencing.

    Conclusions:

    • Standardized tissue acquisition and storage protocols are essential for the success of large-scale genomic projects.
    • The proposed four-star system provides a scalable framework for biobanking and genomic research.
    • Adoption of these standards can advance genomic analyses across diverse species and inspire similar initiatives in other fields.