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Related Experiment Video

Updated: May 12, 2026

Isolation of Adipose Tissue Immune Cells

Isolation of Adipose Tissue Immune Cells

Published on: May 22, 2013

Adipocytes as immune regulatory cells.

Silvana A Vielma¹, Richard L Klein, Corinne A Levingston

¹Department of Otolaryngology, Medical University of South Carolina, Charleston, SC 29425, United States. vielmasa@musc.edu

International Immunopharmacology

|April 17, 2013

Summary

Adipocytes, or fat cells, can regulate immune responses by stimulating conventional immune cells to release various immune mediators. This study reveals adipocytes

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Last Updated: May 12, 2026

Isolation of Adipose Tissue Immune Cells

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Published on: May 22, 2013

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

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Published on: March 6, 2018

Identification and Dissection of Diverse Mouse Adipose Depots

Identification and Dissection of Diverse Mouse Adipose Depots

Published on: July 11, 2019

Area of Science:

Immunology
Cell Biology
Endocrinology

Background:

Obesity is linked to chronic inflammation, with adipocytes producing inflammatory mediators.
The role of adipocytes in immune regulation beyond inflammation is not fully understood.

Purpose of the Study:

To investigate if adipocytes can act as immune regulatory cells.
To determine how adipocyte-conditioned media modulate conventional immune cell functions.

Main Methods:

Fibroblast-derived adipocytes and normal spleen cells were used as a model system.
Adipocyte-conditioned media were applied to spleen cell cultures.
Cytokine and chemokine production by spleen cells was analyzed using various assays.

Main Results:

Adipocyte-conditioned media stimulated spleen cells to release Th1 cytokines (IL-2, IFN-γ, GM-CSF) and inflammatory cytokines (IL-6, TNF-α, IL-9).
Modulation of inhibitory cytokines (IL-10, IL-13, IL-4) and T-cell chemokines (CCL5) was observed.
Adipocytes stimulated IFN-γ production within CD8(+) T-cells, not CD4(+) T-cells.

Conclusions:

Adipocytes possess immune regulatory capabilities, influencing conventional immune cells.
Adipocytes stimulate the release of a broad spectrum of immune mediators from spleen cells.
This suggests a more complex role for adipocytes in immune system modulation than previously recognized.