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Updated: May 12, 2026

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes
Bertrand Colignon1, Martine Raes, Marc Dieu
1Département Sciences du Vivant, Centre wallon de Recherches agronomiques, Gembloux, Belgium.
Abstract:
Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to alleviate comigration associated drawbacks. Spot resolution is strikingly improved following this simple and rapid method and the positive impact on protein and peptide identification from MS/MS data, on the analysis of relative changes in protein abundance, and on the detection of PTM is described.
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