Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-organizing bioceramic granules with wave-dissipating architectures for bone void filling.

Biomaterials·2026
Same author

Three-dimensionally-printed biphasic PCL/<b>β</b>-TCP scaffold with spatially confined GelMA/CS hydrogel for coordinated osteochondral regeneration.

Regenerative biomaterials·2026
Same author

Injectable Silk Fibroin-Puerarin Hydrogels with Tunable Supramolecular Organization as a Potential Platform for Tissue Engineering.

ACS omega·2026
Same author

A Mechanically Adaptive Titanium Scaffold With a Lattice-Modulated Piezocatalytic Coating for Infection Treatment and Bone Regeneration.

Advanced healthcare materials·2026
Same author

Zinc-containing biomaterials for bone disease therapy and tissue repair: Design principles, mechanistic insights, and translational pathways.

Biomaterials·2026
Same author

Hierarchical micro-/nanostructured hydroxyapatite scaffolds promote osteoporotic bone regeneration via activation of hedgehog and HIF-1α signaling.

Bioactive materials·2026

Related Experiment Video

Updated: May 12, 2026

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts
10:32

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

Published on: March 26, 2015

PLDLA/PCL-T Scaffold for Meniscus Tissue Engineering.

Andrea Rodrigues Esposito1, Marlon Moda, Silvia Mara de Melo Cattani

  • 1Department of Materials Engineering, Faculty of Mechanical Engineering, University of Campinas (UNICAMP) , Campinas, Brazil . ; Laboratory of Biomaterials, Faculty of Medicine and Health Sciences, Pontifical Catholic University of Sao Paulo (PUC-SP) , Sorocaba, Brazil .

Bioresearch Open Access
|April 18, 2013
PubMed
Summary

Tissue engineering using fibrochondrocytes seeded on poly(L-co-D,L-lactic acid)/poly(caprolactone-triol) scaffolds promotes meniscus regeneration. This approach aids in repairing joint injuries and protecting articular cartilage from degeneration.

Keywords:
PLDLA/PCL-T scaffoldfibrochondrocyte culturemeniscus regeneration

More Related Videos

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
08:30

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

Published on: April 30, 2014

Establishment and Evaluation of a Sheep Model of Full-thickness Osteochondral Defect
05:23

Establishment and Evaluation of a Sheep Model of Full-thickness Osteochondral Defect

Published on: April 14, 2026

Related Experiment Videos

Last Updated: May 12, 2026

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts
10:32

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

Published on: March 26, 2015

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
08:30

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

Published on: April 30, 2014

Establishment and Evaluation of a Sheep Model of Full-thickness Osteochondral Defect
05:23

Establishment and Evaluation of a Sheep Model of Full-thickness Osteochondral Defect

Published on: April 14, 2026

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Surgery

Background:

  • Meniscal injuries are common and difficult to heal due to the avascular nature of meniscus tissue.
  • Current treatments for meniscal injuries often have limitations.
  • Tissue engineering offers a promising approach for meniscus regeneration.

Purpose of the Study:

  • To evaluate the efficacy of fibrochondrocytes preseeded on poly(L-co-D,L-lactic acid)/poly(caprolactone-triol) (PLDLA/PCL-T) scaffolds for stimulating whole meniscus regeneration.
  • To assess the biocompatibility and regenerative potential of PLDLA/PCL-T scaffolds in a rabbit model.

Main Methods:

  • Porous PLDLA/PCL-T (90/10) scaffolds were fabricated using solvent casting and particulate leaching.
  • Fibrochondrocytes were isolated from rabbit menisci, seeded onto scaffolds, and cultured for 21 days.
  • Implants (cell-free or cell-seeded scaffolds) were surgically placed in rabbit medial knee menisci after total meniscectomy; a control group received no implant.

Main Results:

  • PLDLA/PCL-T scaffolds demonstrated good biocompatibility, with no signs of rejection, infection, or chronic inflammation.
  • Histological analysis at 24 weeks revealed significant fibrocartilaginous tissue formation with mature collagen fibers in cell-seeded scaffold implants compared to cell-free scaffolds.
  • Articular cartilage was preserved, and chondrocyte cell numbers were higher in the polymeric implant groups than in the control group.

Conclusions:

  • The PLDLA/PCL-T 90/10 scaffold supports the formation of fibrocartilaginous tissue, crucial for joint repair.
  • This scaffold shows potential for orthopedic applications, including meniscus regeneration and articular cartilage protection.
  • Cell-seeded scaffolds significantly enhance meniscus regeneration compared to cell-free scaffolds.