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Updated: May 12, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Prediction and identification of Taenia solium oncosphere TSO45-4B antigen FnIII structure domain linear B cell
Yuan-Yuan Wang1, Xiao-Li Wang, Xue-Lian Chang
1Department of Microbiology and Parasitology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bengbu 233030, China.
Objective:
To predict and identify Taenia solium oncosphere TSO45-4B antigen Fn III structure domain linear B cell epitopes.
Methods:
The B cell epitopes of TSO45-4B Fn III structure domain were predicted through the sequence analysis by using bioinformatics online tools and the protein space conformation was predicted by SWISS-MODEL. The peptides were synthesized according to the predicted linear epitopes. The immunoreactivity of sera of cysticercosis patients to the peptides synthesized was tested by using ELISA.
Results:
Two linear B cell epitopes of TSO45-4B Fn III structure domain were predicted, and one of the predicted epitope peptides synthesized could be recognized by the sera of cysticercosis patients.
Conclusion:
Two linear B cell epitopes of TSO45-4B Fn III structure domain are predicted and one of them has been confirmed successfully.