[Determination of six anthraquinone compounds in soil by parallel type dual gradient high performance liquid...

A high performance liquid chromatography (HPLC) was established for the determination of six anthraquinone compounds, 1,8-dinitroanthraquinone, 1,5-dinitroanthraquinone, 1-aminoanthraquinone, anthraquinone, naphthalene, 1,4,5,8-tetrachlorodibenzo anthraquinone, in polluted soil of residential construction. In this method, by using an Ultimate dual gradient liquid chromatograph (DGLC), designing a set of parallel dual system and accepting two same Dikma Diamonsil C18 columns (150 mm x 4.6 mm, 5 microm) to cross-analysis, 41.46% of the analysis time can be saved. By using assisted ultrasonication to the extraction of the six substances in the samples with 50 mL N,N-dimethyl-formamide/1,4-dioxane/methanol (5/20/25, v/ v/v), the average recoveries of the six analytes spiked in nine blank soil samples were 82.7% - 101% with the relative standard deviations (RSDs) of 1.8% - 3.2% and the limits of detection were 0.007 - 0.054 mg/kg, which can reach the limited requirement of the local standard in Beijing of DB11/T811-2011. With the advantages of accessibility, high sensitivity and good reproducibility, this method can be used in the determination of anthraquinone compounds in soil.