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Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
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Quenching effect on gold nano-patterned cardiac troponin I chip by total internal reflection fluorescence microscopy

Seungah Lee1, Seong Ho Kang

  • 1Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.

Talanta
|April 20, 2013
PubMed

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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.