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Y Moliner-Martínez1, R Herráez-Hernández, C Molins-Legua
1Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, C/Dr. Moliner 50, - Burjassot, Valencia E46100, Spain.
Talanta
|April 20, 2013
Summary
This study compares methods for sampling and determining volatile thiols using derivatization with OPA and isoleucine. Different supports were evaluated for trapping and derivatizing thiols, aiding in method selection.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Biochemistry
Background:
- Volatile thiols are important in various fields but challenging to analyze.
- Accurate sampling and determination are crucial for reliable results.
Purpose of the Study:
- To develop and compare strategies for volatile thiol sampling and determination.
- To optimize derivatization conditions and assess storage stability.
Main Methods:
- Air sampling followed by derivatization with o-phthalaldehyde (OPA) and isoleucine in alkaline solution.
- Separation and detection of derivatization products using liquid chromatography and fluorescence detection.
- Evaluation of different trapping supports: impingers, C18 cartridges, and glass fiber filters.
Main Results:
- Optimized derivatization conditions and established storage protocols for thiol derivatives.
- Demonstrated varying analytical performance based on the trapping support used.
- Achieved high selectivity and sensitivity through specific derivatization.
Conclusions:
- The study provides a comparative analysis of different volatile thiol analysis strategies.
- Recommended procedures are presented based on specific analytical requirements and situations.
- The findings contribute to improved methods for volatile thiol monitoring.
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