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Breeding by Design for Functional Rice with Genome Editing Technologies

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Published on: January 3, 2025

Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding.

Nancy Podevin¹, Howard V Davies, Frank Hartung

¹The European Food Safety Authority-EFSA, Via Carlo Magno 1A, Parma, Italy.

Trends in Biotechnology

|April 23, 2013

Summary

New gene editing tools, site-directed nucleases (SDNs), offer advanced plant breeding capabilities beyond conventional methods. While promising for crop improvement, technical and regulatory hurdles remain for widespread commercial adoption.

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Area of Science:

Agricultural Science
Biotechnology
Genetics

Background:

Conventional plant breeding and mutagenesis have been vital for global food security.
Genetically modified plants offer complementary breeding strategies, yet all methods possess inherent limitations.

Purpose of the Study:

To explore how advanced site-directed nuclease (SDN) technologies address limitations in current plant breeding.
To highlight the potential of SDNs for developing novel crop traits.

Main Methods:

Discussion of targeted mutagenesis and DNA insertion techniques utilizing site-directed nucleases (SDNs).
Analysis of the application of SDNs by plant breeding companies for crop trait development.

Main Results:

SDNs represent an evolution in plant breeding, enabling the creation of crops with enhanced traits.
Plant breeding companies are actively utilizing SDNs to develop next-generation crops.

Conclusions:

SDNs offer significant opportunities to overcome limitations in conventional and genetically modified plant breeding.
Technical challenges and regulatory uncertainties surrounding SDNs may impede their commercial implementation in plant breeding.