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Updated: May 12, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
Published on: January 14, 2016
Chromatin modifications and chromatin remodeling during DNA repair in budding yeast
Michael Tsabar1, James E Haber
1Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Waltham, MA 02454-9110, United States.
Abstract:
Double-strand breaks (DSBs) pose a serious threat to genome integrity. Eukaryotes from yeast to humans respond to DSB damage by activating a complex DNA damage response that includes imposing a block to cell cycle progression and the repair of the DSB by one of several pathways. Many of these processes are accompanied by alterations in chromosome and chromatin structure. In this review we focus on the checkpoint responses and DNA repair in the well-studied model organism, the budding yeast Saccharomyces cerevisiae.
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