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Identification of Kinase-substrate Pairs Using High Throughput Screening

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Published on: August 29, 2015

MAP kinases phosphorylate rice WRKY45.

Yoshihisa Ueno¹, Riichiro Yoshida, Mitsuko Kishi-Kaboshi

¹Disease Resistant Crops Research Unit; National Institute of Agrobiological Sciences; Tsukuba, Ibaraki, Japan.

Plant Signaling & Behavior

|April 23, 2013

Summary

Rice disease resistance involves salicylic acid (SA) and WRKY45. This study reveals a mitogen-activated protein kinase (MAPK) cascade regulates WRKY45 mRNA accumulation via phosphorylation, uncovering a key regulatory mechanism.

Keywords:

MAPK disease resistance phosphorylation proteasome rice salicylic acid

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Area of Science:

Plant molecular biology
Plant pathology
Signal transduction

Background:

WRKY45 transcription factor is crucial for salicylic acid (SA)-mediated disease resistance in rice.
SA-activated WRKY45 protein enhances its own mRNA levels, but the regulatory mechanism remains unclear.

Purpose of the Study:

To elucidate the mechanism by which SA-activated WRKY45 protein induces its own mRNA accumulation.
To investigate the role of mitogen-activated protein kinase (MAPK) cascades in this regulatory process.

Main Methods:

Utilized MAPK kinase (MAPKK) inhibitors to assess their effect on WRKY45 transcript levels.
Performed in vitro phosphorylation assays using rice MAPKs (OsMPK4 and OsMPK6) and WRKY45 protein.
Measured the activity of OsMPK6 following SA treatment in rice cells.

Main Results:

MAPKK inhibition suppressed SA-induced increases in WRKY45 mRNA.
OsMPK4 and OsMPK6 were confirmed to phosphorylate WRKY45 protein in vitro.
SA treatment rapidly upregulated the activity of OsMPK6 in rice cells.

Conclusions:

A MAPK cascade is involved in the regulation of WRKY45 mRNA accumulation in rice.
WRKY45 is subject to MAPK-dependent phosphorylation within the SA signaling pathway.
This phosphorylation mechanism is a key component of rice disease resistance regulation.