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Related Experiment Video

Updated: May 12, 2026

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
09:44

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

Published on: December 21, 2015

Aspergillus flavus expressed sequence tags and microarray as tools in understanding aflatoxin biosynthesis.

J Yu1, T E Cleveland, J R Wilkinson

  • 1Food and Feed Safety Research, USDA-ARS Southern Regional Research Center, 1100 Robert E. Lee Blvd., 70124, New Orleans, LA, USA, jiuyu@strcars.usda.gov.

Mycotoxin Research
|April 23, 2013
PubMed
Summary

Researchers identified genes controlling aflatoxin production in Aspergillus flavus using expressed sequence tags (ESTs) and microarrays. This study aims to develop strategies for controlling toxic aflatoxin contamination in agricultural products.

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Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

Published on: April 19, 2024

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Last Updated: May 12, 2026

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
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10:24

Non-destructive SPE-UPLC-based Quantification of Aflatoxins and Stilbenoid Phytoalexins in Single Peanut (Arachis spp.) Seeds

Published on: April 19, 2024

Area of Science:

  • Mycology
  • Molecular Biology
  • Food Safety

Background:

  • Aflatoxins are highly toxic and carcinogenic mycotoxins produced by Aspergillus species.
  • Understanding the genetic basis of aflatoxin production is crucial for controlling contamination.

Purpose of the Study:

  • To identify genes involved in aflatoxin biosynthesis and regulation in Aspergillus flavus.
  • To develop novel strategies for mitigating aflatoxin contamination in agricultural commodities.

Main Methods:

  • Sequencing and annotation of Aspergillus flavus expressed sequence tags (ESTs).
  • Construction and application of microarrays for gene expression profiling.
  • Identification of differentially expressed genes under aflatoxin-producing conditions.

Main Results:

  • Identified 7,218 unique EST sequences, with several genes linked to aflatoxin biosynthesis, regulation, and fungal development.
  • Gene expression profiling revealed hundreds of genes potentially involved in aflatoxin production.
  • Gene knockout experiments are in progress to validate gene functions.

Conclusions:

  • The study provides a comprehensive genetic resource for understanding aflatoxin production.
  • Identified genes offer potential targets for developing control strategies against aflatoxin contamination.
  • Further research will elucidate specific gene functions and their roles in aflatoxin biosynthesis.