T-2 and HT-2 toxin analysis in cereals and cereal products following IAC cleanup and determination via GC-ECD after
1Landesuntersuchungsamt Rheinland-Pfalz, Institut für Lebensmittelchemie, Maximineracht 11a, 54295, Trier, Germany, Paul.Majerus@lua.rlp.de.
Abstract:
The authors present a new and sensitive method for the determination of T-2- und HT-2 Toxin in cereals and cereal products in the low ppb level. A representative part of the cereal sample is extracted with a mixture of methanol-water (90:10) and the extract is cleaned on the commercially available immunoaffinity column T-2test™ (IAC), eluted with methanol, derivatized by pentafluorpropionic anhydride (PFPA) and measured on a GC-ECD. The method has been successfully validated on wheat, rye and oats. The recovery rates with wheat and rye endowed on a level of 50 ppb and with 85 ppb naturally contaminated oats were 71-115% with a coefficient of variation of 5.7-19.5%. The detection limits of the method with a signal to noise level of 3:1 were 1.5-2.3 μg/kg for HT-2 and 1.1-1.7 μg/kg for T-2 toxin.
