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Updated: May 12, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
Published on: September 23, 2021
A DOTAM-based paraCEST agent favoring TSAP geometry for enhanced amide proton chemical shift dispersion and
Todd K Stevens1, Mark Milne, Adam A H Elmehriki
1Centre for Functional and Metabolic Mapping, Robarts Research Institute, London, Ontario, Canada.
Abstract:
The Tm(3+) chelate of DOTAM [1,4,7,10-tetrakis(carbamoylmethyl)-1,4,7,10-tetraazacyclododecane] possessing sterically demanding t-butyl amide substitution favors TSAP geometry. This chelate displayed a paraCEST signal associated with the highly shifted amide proton signal at approximately -100 ppm that was beyond the frequency of macromolecule magnetization transfer. This signal also displayed high temperature dependence (0.57 ppm °C(-1)) in the range of 35-42 °C and at neutral pH.
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