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Updated: May 12, 2026
In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
Published on: May 2, 2008
In vivo determination of vitamin d function using transgenic mice carrying a human osteocalcin luciferase reporter
Tomoko Nakanishi1, Rumiko Saito, Makoto Taniguchi
1Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago, Nishicho 86, Tottori 683-8503, Japan. tomo@med.tottori-u.ac.jp
Abstract:
Vitamin D is an essential factor for ossification, and its deficiency causes rickets. Osteocalcin, which is a noncollagenous protein found in bone matrix and involved in mineralization and calcium ion homeostasis, is one of the major bone morphogenetic markers and is used in the evaluation of osteoblast maturation and osteogenic activation. We established transgenic mouse line expressing luciferase under the control of a 10-kb osteocalcin enhancer/promoter sequence. Using these transgenic mice, we evaluated the active forms of vitamins D2 and D3 for their bone morphogenetic function by in vivo bioluminescence. As the result, strong activity for ossification was observed with 1 α ,25-hydroxyvitamin D3. Our mouse system can offer a feasible detection method for assessment of osteogenic activity in the development of functional foods and medicines by noninvasive screening.
