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Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
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The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.

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Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
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Published on: November 11, 2016

Deciphering a serum peptide activity associated with biologic clock.

Yu Chen1, Ho Wing Shing

  • 1Department of Pharmacology & Therapeutics, McGill University, Montreal, Quebec H3G 1Y6, Canada. yolanda009@gmail.com

Protein and Peptide Letters
|April 24, 2013
PubMed
Summary
This summary is machine-generated.

A human serum protein deficiency linked to sleep disorders was identified as RBM4 (RNA binding protein 4), also known as human lark protein. This protein is crucial for cellular processes and may reveal a novel sub-nuclear pathway.

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Area of Science:

  • Chronobiology
  • Molecular Biology
  • Human Genetics

Background:

  • Circadian rhythms, approximately 24-hour cycles in biological processes, are fundamental across diverse organisms.
  • These endogenous rhythms require adjustment to environmental cues and stable periodicity despite cellular activity fluctuations.
  • Specific cellular proteins are implicated in the regulation and maintenance of circadian rhythms.

Purpose of the Study:

  • To investigate the molecular basis of a serum protein deficiency observed in human subjects with sleep disorders.
  • To identify the protein associated with circadian rhythm regulation and its potential role in sub-nuclear localization.

Main Methods:

  • Serum protein analysis in human subjects diagnosed with sleep disorders.
  • N-terminal sequence analysis of a deficient peptide.
  • BLAST sequence alignment using NCBI databases to identify the protein sequence.

Main Results:

  • A deficiency in a specific serum protein was detected in individuals with sleep disorders.
  • Sequence analysis identified the protein as potentially related to human lark protein (Hlark), which is RBM4 (RNA binding protein 4).
  • RBM4, a splicing regulator, is essential for embryonic development and targets speckles, suggesting a novel sub-nuclear pathway.

Conclusions:

  • The human lark protein (Hlark) is likely identical to RBM4, a crucial splicing regulator.
  • RBM4's localization in speckles, nucleoli, and peri-nucleolar clusters supports a novel sub-nuclear targeting pathway.
  • This finding links RBM4/Hlark to circadian rhythm regulation and sleep disorders.