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Related Concept Videos

Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
Chromatographic Resolution01:15

Chromatographic Resolution

In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

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Updated: May 12, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Reliable peak selection for multisample analysis with comprehensive two-dimensional chromatography.

Stephen E Reichenbach1, Xue Tian, Akwasi A Boateng

  • 1University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0115, United States. reich@cse.unl.edu

Analytical Chemistry
|April 24, 2013
PubMed
Summary
This summary is machine-generated.

Analyzing complex samples with comprehensive two-dimensional chromatography is challenging. A new Consistent Cliques Method (CCM) automates peak matching for reliable comparative analysis across many samples.

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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Chemometrics

Background:

  • Comprehensive two-dimensional chromatography (GCxGC) is vital for analyzing complex mixtures.
  • Matching chromatographic features across numerous samples is a significant analytical challenge.
  • Existing pairwise peak matching methods often yield incomplete or inconsistent results.

Purpose of the Study:

  • To develop an automated postprocessing method for improving chromatographic feature matching.
  • To address the limitations of incomplete and inconsistent peak matches in large sample sets.
  • To enable reliable comparative analysis and data alignment for complex chemical mixtures.

Main Methods:

  • The Consistent Cliques Method (CCM) was developed for postprocessing pairwise peak matching results.
  • CCM constructs a graph of all peaks and pairwise matches across chromatograms.
  • Maximal cliques are identified and combined to extract reliable, consistently matched features.

Main Results:

  • CCM effectively addresses incomplete and inconsistent peak matching issues.
  • The method selects chromatographic peaks that reliably correspond across multiple samples.
  • Experimental validation using GCxGC-MS of bio-oils demonstrated CCM's utility.

Conclusions:

  • The Consistent Cliques Method (CCM) provides a robust foundation for comparative analysis of complex chemical mixtures.
  • CCM facilitates direct comparison of relative compositions and alignment of chromatographic data.
  • This automated approach enhances the reliability and scalability of analyzing large sample sets.