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Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Atomic Absorption Spectroscopy: Atomization Methods01:25

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...
Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

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Updated: May 12, 2026

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
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Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

Laser ablation in analytical chemistry.

Richard E Russo1, Xianglei Mao, Jhanis J Gonzalez

  • 1Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA. rerusso@lbl.gov

Analytical Chemistry
|April 26, 2013
PubMed
Summary
This summary is machine-generated.

This review updates the physics of laser ablation in microchemical analysis. It covers current research, applications like Laser-Induced Breakdown Spectroscopy (LIBS) and Laser Ablation Molecular Spectrometry (LAMIS), and future directions.

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Last Updated: May 12, 2026

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Area of Science:

  • Analytical Chemistry
  • Physics

Background:

  • This manuscript builds upon a 2002 Analytical Chemistry feature article on the physics of laser ablation.
  • It addresses the evolution of the field since the previous publication.

Purpose of the Study:

  • To discuss current fundamental research in laser ablation for microchemical analysis.
  • To review applications involving in-situ photon detection (LIBS, LAMIS) and particle collection (ICP).
  • To outline future directions for laser ablation technology.

Main Methods:

  • Review of fundamental research in laser ablation.
  • Analysis of applications using Laser-Induced Breakdown Spectroscopy (LIBS) and Laser Ablation Molecular Spectrometry (LAMIS).
  • Examination of techniques involving Inductively Coupled Plasma (ICP) for particle excitation.

Main Results:

  • Identifies key current issues in fundamental laser ablation research.
  • Highlights the capabilities and advancements in LIBS, LAMIS, and ICP-based applications.
  • Provides insights into emerging trends and potential future developments.

Conclusions:

  • Laser ablation remains a critical technique in microchemical analysis.
  • Continued research and technological advancements are expanding its applications.
  • The field is poised for further innovation in detection and analysis.