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Updated: May 12, 2026

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Published on: November 5, 2014
A novel fluorescent "Turn-Off/Turn-On" system for the detection of acid phosphatase activity
Pu Guo1, Shengyong Yan, Yimin Zhou
1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P.R. China.
Abstract:
Acid phosphatase (ACP) can be sensitively, conveniently and efficiently detected via a new fluorescent "Turn-Off/Turn-On" system. Inexpensive (NaPO(3))(6) is carefully introduced into this system as a quencher of the aggregation-caused quenching (ACQ) probe. In our method, the limit of detection (LOD) is quite low for detecting ACP, which is an important biomarker and indication of several diseases.
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