Determination of dobutamine hydrochloride by enzymatic catalytic spectrofluorimetry

A highly sensitive and simple spectrofluorimetric method for the determination of dobutamine hydrochloride based on its inhibitory effect on the hemoglobin-catalyzed reaction of H2 O2 and l-tyrosine was developed. The relationship between the concentration of dobutamine hydrochloride and the fluorescence quenching (ΔF) of the system is linear under the optimal experimental conditions. The calibration graph is linear in the range 2.00 × 10(-7) to 3.00 × 10(-6) g/mL with a limit of detection of 4.83 × 10(-9) g/mL. This method can be used for the determination of dobutamine hydrochloride in its pharmaceutical formulations and in urine with satisfactory results.