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Related Experiment Video

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Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
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MAPK establishes a molecular context that defines effective training patterns for long-term memory formation.

Gary T Philips1, Xiaojing Ye, Ashley M Kopec

  • 1Center for Neural Science, New York University, New York, New York 10003, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|April 26, 2013
PubMed
Summary

Spaced training enhances long-term memory (LTM) by requiring protein synthesis-dependent nuclear MAPK activity between trials. This molecular signaling is crucial for effective memory formation intervals.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Memory Research

Background:

  • Spaced training enhances long-term memory (LTM) formation.
  • Interactions between training trials are critical for LTM.
  • The role of ERK/MAPK in mediating these intertrial interactions is largely unknown.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying intertrial interactions in LTM formation.
  • To determine if MAPK signaling is required for intertrial interactions during memory consolidation.
  • To elucidate the role of MAPK kinetics in effective training intervals for LTM.

Main Methods:

  • Utilized a novel two-trial training paradigm in Aplysia to induce LTM.
  • Investigated delayed, protein synthesis-dependent nuclear MAPK activity following the first training trial.
  • Examined the recruitment of CREB kinase and ApC/EBP in response to MAPK activation.

Main Results:

  • The first training trial triggers delayed, protein synthesis-dependent nuclear MAPK activity.
  • This MAPK activity establishes a molecular context essential for LTM induction.
  • MAPK signaling is demonstrated as a requirement for intertrial interactions in memory formation.

Conclusions:

  • MAPK plays a critical role in mediating intertrial signaling necessary for LTM.
  • The kinetics of MAPK activation dictates optimal training intervals for memory.
  • This study provides the first evidence for MAPK's requirement in intertrial interactions during memory consolidation.