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Published on: June 5, 2019
Human platelet-specific antigen frequencies in Indonesian population
Asmarinah1, R Dharma, N K Ritchie
1Department of Medical Biology, Faculty of Medicine, Universitas Indonesia. asmarinah.si@gmx.de
Transfusion Medicine (Oxford, England)
|April 27, 2013
Summary
Alloantibodies against human platelet antigens (HPAs) cause alloimmune thrombocytopenia. This study determined HPA allele frequencies in Indonesian donors, finding low risks for HPA-1, -2, and -6 incompatibilities, crucial for managing transfusion refractoriness and NAIT.
Area of Science:
- Immunogenetics
- Transfusion Medicine
- Population Genetics
Background:
- Alloantibodies against human platelet antigens (HPAs) are implicated in alloimmune thrombocytopenia, including platelet transfusion refractoriness (PTR) and neonatal alloimmune thrombocytopenia (NAIT).
- Transfusion of HPA-compatible platelets is critical for managing these conditions.
Purpose of the Study:
- To determine the allele frequencies of major HPA systems in Indonesian blood donors.
- To establish Indonesia's first HPA-typed donor registry.
Main Methods:
- Genotyping of 500 Indonesian blood donors for HPA-1 to HPA-6 and HPA-15 alleles.
- Utilized the polymerase chain reaction sequence-specific primer (PCR-SSP) method.
Main Results:
- Gene frequencies for rare alleles HPA-1b, -2b, -3b, -4b, -5b, -6b, and -15b were determined.
- No donors homozygous for HPA-1b, -2b, and -6b were identified, suggesting low alloimmunization risk for these systems.
- Alloimmunization against HPA-3, -4, -5, and -15 systems is anticipated.
Conclusions:
- The study highlights the need for an HPA-genotyped registry, particularly for donors homozygous for HPA-1b, -2b, and -6b.
- This registry is essential for optimizing the management of PTR patients and infants with NAIT.
