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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
Th2-type innate immune responses mediated by natural helper cells
1Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan. koyasu@z3.keio.jp
Annals of the New York Academy of Sciences
|April 27, 2013
Summary
Natural helper (NH) cells are innate immune cells that produce T helper cell type 2 (Th2) cytokines. These cells are crucial for antihelminth immunity and allergic diseases like asthma.
Area of Science:
- Immunology
- Innate Immunity
- Lymphocyte Biology
Background:
- Natural helper (NH) cells are a recently discovered population of innate lymphocytes.
- NH cells are found in fat-associated lymphoid clusters (FALCs).
- They constitutively produce T helper cell type 2 (Th2) cytokines without stimulation.
Purpose of the Study:
- To characterize the function and cytokine production of Natural helper (NH) cells.
- To investigate the role of NH cells in immune responses.
- To understand the involvement of NH cells in antihelminth immunity and allergic diseases.
Main Methods:
- Identification and characterization of NH cells.
- Analysis of cytokine production in response to interleukins (IL-2, IL-25, IL-33).
- Assessment of NH cell function in supporting B cell activity.
Main Results:
- NH cells respond to IL-2 combined with IL-25 or IL-33 by producing significant amounts of Th2 cytokines.
- NH cells support the self-renewal of B1 cells and IgA production.
- IL-5 and IL-13, produced by activated NH cells, can induce eosinophilia and goblet cell hyperplasia.
Conclusions:
- NH cells are key players in Th2-mediated immune responses.
- These cells are important for antihelminth immunity and contribute to allergic diseases such as asthma.
- NH cells represent a significant target for therapeutic interventions in immune-related disorders.
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