Long-term RNA persistence in postmortem contexts.

Area of Science:

• Molecular Biology
• Biochemistry
• Forensic Science

Background:

• Ribonucleic acids (RNA) are typically viewed as unstable and rapidly degrading molecules.
• Recent findings indicate significant long-term persistence of RNA across diverse tissues and timeframes (days to centuries).
• This persistence has opened new avenues for RNA utilization in various scientific disciplines.

Purpose of the Study:

• To review and outline the molecular mechanisms contributing to long-term RNA survival postmortem.
• To provide a comprehensive overview of RNA decay mechanisms, addressing a gap in existing literature.
• To present examples of RNA persistence in forensic, archival, and archaeological contexts.

Main Methods:

• Literature review of existing studies on RNA postmortem instability and decay.
• Analysis of molecular factors potentially safeguarding RNA against degradation.
• Compilation of case studies demonstrating long-term RNA survival.

Main Results:

• Several plausible molecular mechanisms exist that protect RNA from degradation.
• Documented instances of RNA survival range from days to centuries in various sample types.
• Specific examples highlight RNA persistence in forensic investigations, historical archives, and archaeological finds.

Conclusions:

• Understanding RNA decay mechanisms is essential for predicting its long-term stability.
• Further research into RNA stabilization is crucial for maximizing its utility in diverse scientific fields.
• The review provides a foundation for future studies on RNA preservation and analysis.