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Published on: September 1, 2019
Analysis of chromosome damage for biodosimetry using imaging flow cytometry
L A Beaton1, C Ferrarotto, B C Kutzner
1Consumer and Clinical Radiation Protection Bureau, Health Canada, 775 Brookfield Road, Postal Locator 6303B, Ottawa, ON, Canada.
Mutation Research
|April 27, 2013
Summary
The dicentric chromosome assay (DCA) is now adaptable to imaging cytometry, enabling faster, automated radiation biodosimetry. This advancement improves high-throughput analysis for mass casualty events.
Area of Science:
- Cytometry
- Radiation Biology
- Genetics
Background:
- The dicentric chromosome assay (DCA) is the gold standard for radiation biodosimetry.
- Current DCA methods require manual scoring, limiting throughput.
- Emergency response requires rapid dose estimation.
Purpose of the Study:
- To adapt the DCA to an automated imaging cytometry method.
- To maintain microscopic sensitivity while increasing throughput.
- To enable rapid biological dose determination for mass casualty events.
Main Methods:
- Peripheral blood mononuclear cells (PBMC) were isolated from irradiated blood.
- Cells were cultured, chromosomes isolated, and stained with propidium iodide and a centromere marker.
- Analysis was performed on an ImageStream(×) imaging cytometer.
Main Results:
- Individual chromosomes were identified, and mono- and dicentric chromosomes differentiated.
- A dose-response curve was generated using imaging cytometry.
- Preliminary results show comparability to traditional microscope scoring.
Conclusions:
- Imaging cytometry enables rapid, automated analysis of fluorescently labeled chromosomes.
- Adapting the DCA to imaging cytometry offers high-throughput potential for mass casualty events.
- This technology enhances radiation biodosimetry capabilities.
