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Updated: Jul 15, 2026

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Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Rapid analysis of non-esterified fatty acids as methyl esters from different biological specimens by gas
1Institute of Pediatrics, Department of Mass Spectrometry, Graz, Austria.
Journal of Chromatography
|April 6, 1990
Abstract:
A rapid gas chromatographic method for the determination of medium-chain and long-chain free fatty acids (C14:0 to C24:0 fatty acids) from different biological specimens is presented. After a rapid one-step transesterification method in methanol-acetyl chloride (50:1, v/v), fatty acid methyl esters were extracted into n-hexane and analysed on a 15-m Durabond-Wax column within a 12-min chromatographic run. The detection limit is 500 pg per injection.

