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Updated: May 11, 2026

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
Published on: July 10, 2018
Striatum-specific expression of Cre recombinase using the Gpr88 promoter in mice
Chihiro Hisatsune1, Naoko Ogawa, Katsuhiko Mikoshiba
1Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute (BSI) 2-1 Hirosawa, Wako City, Saitama, 351-0198, Japan, chihiro@brain.riken.jp.
Abstract:
We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked chloramphenicol acetyltransferase gene, and examined the Cre activity in the Tg mouse brains by assessing β-galactosidase activity. Cre activity was specifically detected in the caudate-putamen, nucleus accumbens, and olfactory tubercle of the Gpr88-Cre Tg mouse brain. Medium spiny neurons within the caudate-putamen exhibited Cre activity. Thus, Gpr88-Cre Tg mice could be a useful tool for analyzing the function of the basal ganglia by using Cre/loxP systems.
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