Related Experiment Video
Updated: May 11, 2026
06:01
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
Published on: January 7, 2019
Analysis of secreted proteins
Valeria Severino1, Annarita Farina, Angela Chambery
1Second University of Naples, Caserta, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|April 30, 2013
Summary
Analyzing cell secretomes, the proteins cells release, aids understanding biological processes and disease. This research outlines methods for secretome profiling, crucial for discovering biomarkers and therapeutic targets.
Area of Science:
- Cellular Biology
- Proteomics
- Biochemistry
Background:
- Biological processes are regulated by signaling pathways involving secreted proteins.
- Understanding extracellular signaling molecules is key to cellular regulatory networks.
- Cell secretome profiling is vital for biomarker discovery and therapeutic target identification.
Purpose of the Study:
- To describe a workflow for analyzing proteins secreted by cultured cells.
- To discuss critical factors in secretome preparation and mass spectrometry analysis.
- To present an overview of quantitative approaches and validation strategies for secretome biomarkers.
Main Methods:
- Utilizing proteomic and mass spectrometry platforms for secretome profiling.
- Analyzing conditioned media from cultured cell lines and primary cells.
- Applying quantitative LC-MS-based approaches and computational tools for data analysis.
Main Results:
- Secretome analysis presents technical challenges impacting mass spectrometry.
- A typical workflow for analyzing secreted proteins is detailed.
- Strategies for validating potential secretome biomarkers are discussed.
Conclusions:
- Secretome profiling offers insights into cellular regulatory networks.
- Effective secretome analysis requires careful consideration of cell culture and preparation.
- This research provides a framework for advancing biomarker and therapeutic target discovery.
Related Concept Videos
01:33Overview of Secretory Vesicles
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
01:15Western Blotting
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...