Related Experiment Video
Updated: May 11, 2026

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
Published on: May 8, 2016
Chemokine-dependent signaling pathways in the peripheral nervous system
1Neuromuscular Immunopathology Research Laboratory, Department of Neurology, Baylor College of Medicine, Houston, TX, USA.
This study introduces novel methods to investigate chemokine signaling in peripheral nerve inflammation. Researchers developed a human blood-nerve barrier model and a mouse model of experimental autoimmune neuritis to explore immune cell trafficking.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Chemokines and G-protein-coupled receptors are crucial for immune responses and nerve development.
- Dysregulated chemokine signaling in the peripheral nervous system contributes to nerve inflammation.
- Studying these pathways requires robust in vitro and in vivo models.
Purpose of the Study:
- To present reliable methods for studying chemokine-dependent signaling in peripheral nerves.
- To introduce a flow-dependent human leukocyte-blood-nerve barrier (BNB) trafficking assay.
- To describe the induction of experimental autoimmune neuritis (EAN) in mice for studying nerve inflammation.
Main Methods:
- Development of an in vitro human blood-nerve barrier (BNB) model.
- Establishment of a severe murine experimental autoimmune neuritis (EAN) model.
- Implementation of a flow-dependent human leukocyte trafficking assay across the BNB.
Main Results:
- The described methods provide reliable tools for studying leukocyte trafficking and chemokine signaling.
- The EAN model allows for the study of acute peripheral nerve inflammation.
- The BNB assay enables investigation of immune cell interactions at the blood-nerve interface.
Conclusions:
- The developed human in vitro BNB model and murine EAN model are valuable for studying neuroinflammation.
- These tools facilitate research into pro-inflammatory chemokine-dependent signaling pathways in peripheral nerves.
- Understanding these mechanisms is key to addressing neuropathic conditions.
Related Concept Videos
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Parasympathetic Signaling
The effects of...
NF-kB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Diversity in Cell Signaling Responses
Graded and Abrupt Responses
Some signaling systems generate...
Overview of Cell Signaling
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...