Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Spectrophotometry: Introduction

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Parameter influence on the mechanical properties of FFF printed PLA/copper for EV lightweight dashboard analysis.

Scientific reports·2025

Same author

ROC study and SUV threshold using quantitative multi-modal SPECT for bone imaging.

European journal of hybrid imaging·2021

Same author

Frozen elephant trunk in acute type A aortic dissection: surgical techniques and the extracorporeal circuit management.

Indian journal of thoracic and cardiovascular surgery·2021

Same author

Click synthesis of 1,2,3-triazole based imidazoles: Antitubercular evaluation, molecular docking and HSA binding studies.

Bioorganic & medicinal chemistry letters·2021

Same author

Risk factors for Cesarean delivery in pregnancy with small-for-gestational-age fetus undergoing induction of labor.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2019

Same author

Spectrophotometric estimation of risperidone in tablets.

Indian journal of pharmaceutical sciences·2014

Search research articles

Related Experiment Video

Updated: May 11, 2026

Formation of Dispersible Taohong Siwu Tablets

Formation of Dispersible Taohong Siwu Tablets

Published on: February 3, 2023

Spectrophotometric estimation of azithromycin in tablets.

B K Jayanna¹, G Nagendrappa, Arunkumar

¹Department of Chemistry, B. N. M. Institute of Technology, 12th Main Road, 27th Cross, Banashankari II Stage, Bangalore-560 070, India.

Indian Journal of Pharmaceutical Sciences

|April 30, 2013

Summary

A new visible spectrophotometric method accurately estimates azithromycin in tablets. This cost-effective technique utilizes potassium permanganate reduction, offering a sensitive and precise analytical approach for pharmaceutical quality control.

Keywords:

Azithromycin potassium permanganate spectrophotometry tablets

More Related Videos

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

Published on: October 6, 2022

Related Experiment Videos

Last Updated: May 11, 2026

Formation of Dispersible Taohong Siwu Tablets

Formation of Dispersible Taohong Siwu Tablets

Published on: February 3, 2023

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation

Published on: August 1, 2018

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

Published on: October 6, 2022

Area of Science:

Analytical Chemistry
Pharmaceutical Analysis
Spectrophotometry

Background:

Accurate quantification of azithromycin in pharmaceutical formulations is crucial for ensuring therapeutic efficacy and patient safety.
Existing analytical methods may present limitations in terms of cost, sensitivity, or complexity.
Development of simple and economical analytical techniques is essential for routine quality control in pharmaceutical industries.

Purpose of the Study:

To develop and validate a simple, sensitive, accurate, and economical visible spectrophotometric method for the estimation of azithromycin.
To assess the applicability of the developed method for azithromycin determination in tablet formulations.
To evaluate the method's performance regarding interference and agreement with labeled quantities.

Main Methods:

Visible spectrophotometry utilizing the reduction of potassium permanganate in an alkaline medium by azithromycin.
Measurement of the decrease in absorbance of potassium permanganate at 547 nm.
Quantification of azithromycin within the concentration range of 2-20 μg/ml.

Main Results:

The developed method demonstrated good linearity, sensitivity, accuracy, and precision for azithromycin estimation.
No significant interference was observed from common excipients found in azithromycin tablets.
The results obtained from tablet analysis showed good agreement with the labeled drug content.

Conclusions:

The proposed visible spectrophotometric method is suitable for the accurate and economical determination of azithromycin in tablet formulations.
This method offers a reliable alternative for routine quality control analysis of azithromycin.
The simplicity and sensitivity of the method make it advantageous for pharmaceutical analysis.