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[Research the extraction and detection method of gastrodin content in Gastrodia elata]
Ding-Qiao Xu1, Jian-Jun Zhou, Yi-Hong Liu
1School of Biological Science and Engineering, Shaanxi University of Technology/The Center of Gastrodia elata B1. and Dogwood Engineering in Shaanxi, Hanzhong 723000, China. xdq19881120@163.com
Objective:
To improve an extraction and determination method for gastrodin in Gastrodia elata.
Methods:
On the basis of single factor experiment, used the method of hot water reflux extraction and applied the response surface methodology to determine the best conditions for extracting gastrodin and using HPLC test.
Results:
Under the boiling water condition, pure water as solvent, the solid-liquid ratio was 1:37,extraction 2 times and extraction time 126 min per times,the content of gastrodin was 0.377%. Comparison with the 2010 edition of the China pharmacopoeia of gastrodin extracted in Gastrodia elata, the result showed the gastrodin content was increased by 2. 12 times.
Conclusion:
This extraction method of gastrodin dissolution rate is high and can reflect the real content of gastrodin of Gastrodia elata.