Cholesterol reduction from milk using β-cyclodextrin immobilized on glass

Area of Science:

• Materials Science
• Biochemistry
• Food Science

Background:

• Cholesterol removal from milk is crucial for health and food processing.
• Beta-cyclodextrin (β-CD) is known for its ability to complex with cholesterol.
• Immobilizing β-CD on solid surfaces can enhance its reusability and efficiency.

Purpose of the Study:

• To develop a stable, functionalized glass surface using β-CD for cholesterol reduction in milk.
• To evaluate the efficiency and reusability of the immobilized β-CD system.
• To investigate the long-term stability of the β-CD monolayer.

Main Methods:

• Chemical modification of β-CD to β-CD-undecenyl ether.
• Covalent attachment of modified β-CD to a glass surface.
• Characterization using static water contact angle and X-ray photoelectron spectroscopy (XPS).
• Cholesterol extraction experiments in milk at 25°C, 170 rpm for 4 hours.
• Repeated use testing for 10 cycles with XPS analysis after cycles.

Main Results:

• Successful formation of an excellent β-CD monolayer on the glass surface confirmed by contact angle and XPS.
• Achieved 73.6% cholesterol extraction from milk in 4 hours, a record for immobilized β-CD on solid surfaces.
• Demonstrated high reusability over 10 cycles with consistent cholesterol reduction (72±2%).
• XPS analysis confirmed no degradation of the β-CD monolayer after 10 cycles.

Conclusions:

• The developed β-CD immobilized on glass presents a highly efficient and stable system for cholesterol removal from milk.
• The unique structure of β-CD contributes to the monolayer's flexibility, efficiency, and long-term stability.
• This method offers a promising approach for practical applications in food processing and health-related industries.