Polyionic and cysteine-containing fusion peptides as versatile protein tags

Area of Science:

• Biotechnology
• Molecular Biology
• Protein Engineering

Background:

• Recombinant protein production is crucial for medical and biotechnological applications.
• Protein fusion tags are essential tools for protein expression, purification, and functionalization.

Purpose of the Study:

• To summarize the utility of polyionic fusion peptides as protein tags.
• To highlight their applications in protein expression, purification, refolding, and coupling.

Main Methods:

• Utilizing positively or negatively charged polyionic fusion peptides with or without cysteine.
• Applying these tags for protein expression and purification.
• Employing them for matrix-assisted refolding of aggregated proteins.
• Using them for coupling proteins to matrices or other proteins.
• Designing cysteine-containing polyionic fusion peptides for immunotoxin development.

Main Results:

• Polyionic fusion tags demonstrate versatility in protein handling and modification.
• Cysteine-containing tags were successfully used in immunotoxin design.
• These tags serve as multifunctional modules in protein technology.

Conclusions:

• Polyionic fusion tags are valuable tools in protein technology.
• Their applications span expression, purification, refolding, and bioconjugation.
• They represent a multifunctional module for diverse biotechnological purposes.