[Immunoelectrophoretic identification of proteins separated by zonal electrophoresis and isoelectric focusing]

Modified procedure of cross immunoelectrophoresis is developed, which involved zonal polyacrylamide gel electrophoresis and isoelectric focusing in borate polyol and ampholine pH gradients for the first-dimension separation of proteins. Immunoelectrophoretic detection of proteins is suitable for evaluation of their molecular mass, isoelectric points and heterogeneity as well as for estimation of various protein fractions concentrations.