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An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
Published on: July 6, 2019
Note: Submicrometer-precision sample holder for accurate re-positioning of samples in scanning force microscopy
José Abad1, Juan Francisco González Martínez, Jaime Colchero
1Instituto Universitario de Investigación en Óptica y Nanofísica (IUOyN), Campus Espinardo, Universidad de Murcia, E-30100 Murcia, Spain. jose.abad@upct.es
The Review of Scientific Instruments
|May 3, 2013
Summary
Two new sample holders for Scanning Force Microscopy (SFM) offer submicrometer precision for automatic sample positioning. This simplifies ex situ manipulation and imaging without optical aids.
Area of Science:
- Materials Science
- Nanotechnology
- Microscopy
Background:
- Scanning Force Microscopy (SFM) requires precise sample positioning for accurate imaging.
- Current methods for sample manipulation and re-allocation in SFM can be complex and time-consuming.
- Integration of sample holders with piezoelectric scanners is crucial for enhanced control.
Purpose of the Study:
- To develop simple, compact, and high-precision sample holders for SFM systems.
- To enable automatic sample position recovery within submicrometer accuracy.
- To facilitate ex situ sample manipulation and subsequent SFM imaging of the same region without optical assistance.
Main Methods:
- Design and fabrication of two novel sample holder prototypes.
- Integration of kinematic mounting principles or self-adjusting mechanisms.
- Testing of sample position recovery accuracy and repeatability.
Main Results:
- Achieved automatic sample position recovery within approximately 100 nm.
- Demonstrated successful integration into a standard SFM system.
- Validated the ability to perform ex situ manipulation and re-image the same sample area without optical microscopes or positioning marks.
Conclusions:
- The developed sample holders provide a significant improvement in ease of use and precision for SFM.
- These holders streamline workflows by eliminating the need for tedious re-allocation and optical alignment.
- The designs offer a practical solution for researchers requiring accurate sample handling in SFM applications.
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