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Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
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Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

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Ultrasensitive detection of proteins on Western blots with semiconducting polymer dots

Fangmao Ye1, Polina B Smith, Changfeng Wu

  • 1Department of Chemistry, University of Washington, Seattle, WA 98195, USA.

Macromolecular Rapid Communications
|May 3, 2013
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...