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Updated: May 11, 2026
Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
Published on: July 15, 2016
Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos
Sarah E Webb1, Andrew L Miller
1Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China. barnie@ust.hk
Abstract:
The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca(2+) to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.
