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Detection of Rotavirus Infection in Bovine Calves by RNA-PAGE and RT-PCR

Sanjay Singh Basera1, Rashmi Singh, N Vaid

  • 1Department of Veterinary Microbiology, College of Veterinary and Animal Science, G. B. Pant University of Agriculture and Technology, Pantnagar, U. S. Nagar, Uttarakhand India.

Indian Journal of Virology : an Official Organ of Indian Virological Society
|May 3, 2013
PubMed

Keywords:
Bovine calvesDetectionGroup A rotavirusRNA-PAGERT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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