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Growth factor release from platelet concentrates: analytic quantification and characterization for clinical
C Durante1, F Agostini, L Abbruzzese
1Dipartimento di Ricerca Traslazionale, S.O.S. Dipartimentale Trattamento Cellule Staminali per le Terapie Cellulari, Centro di Riferimento Oncologico, IRCCS, Aviano, PN, Italy.
Vox Sanguinis
|May 4, 2013
Summary
Growth factor solutions from whole blood show higher concentrations than those from platelet apheresis. Release kinetics and yields can be modulated by controlling temperature, time, and cell lysis methods.
Area of Science:
- Biotechnology
- Hematology
- Biochemistry
Background:
- Clinical applications of growth factor-rich plasma necessitate rigorous biochemical control.
- Understanding growth factor release from platelet products is crucial for therapeutic efficacy.
Purpose of the Study:
- To quantify and modulate growth factor concentrations in solutions derived from platelet apheresis and whole blood.
- To investigate the impact of different activation methods on growth factor release.
Main Methods:
- Growth factor concentrations (FGF, PDGF, TGF-β, VEGF, EGF) were measured in platelet apheresis products and whole blood concentrates.
- Activation methods included CaCl2 incubation at 40°C, varying incubation temperatures (22°C vs. 40°C) and times (10' vs. 30'), and freeze-thaw cycles.
Main Results:
- Whole blood-derived solutions exhibited 25-60% higher growth factor concentrations compared to platelet apheresis.
- Early release (5-10 min) of VEGF, TGF-β, and PDGF was observed; FGF and EGF release occurred later (>30 min).
- Incubation at 40°C/10' significantly increased VEGF and decreased EGF/PDGF-BB compared to 22°C/30'. Cell lysis (shock) dramatically altered specific growth factor yields.
Conclusions:
- Platelets from both apheresis and whole blood are sources of multiple growth factors.
- Incubation time, temperature, and cell lysis are key modulators of growth factor release profiles.
