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Published on: April 29, 2011
Expression pattern of protein kinase C ϵ during mouse embryogenesis
Sergio Carracedo1, Ursula Braun, Michael Leitges
1Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, Oslo, N-0349, Norway. s.c.huroz@biotek.uio.no
BMC Developmental Biology
|May 4, 2013
Summary
Protein kinase C epsilon (PKCϵ) is highly expressed in developing mouse hearts and ganglia. Despite its expression, PKCϵ-deficient mice show no obvious developmental defects, suggesting functional redundancy.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- Protein kinase C epsilon (PKCϵ) is part of the novel PKC subfamily, known for roles in wound healing and cancer.
- PKCϵ is calcium-independent and diacylglycerol-dependent.
Purpose of the Study:
- To investigate the expression pattern of PKCϵ during murine midgestation.
- To expand the understanding of PKCϵ's role in embryonic development.
Main Methods:
- Utilized LacZ reporter gene expression analysis.
- Employed immunostaining techniques to visualize PKCϵ localization.
Main Results:
- Highest PKCϵ expression observed in the heart during early stages and in ganglia in later stages.
- Expression also noted in somites, bone, stomach, kidney, and blood vessels.
- PKCϵ-deficient mice did not exhibit apparent embryonic developmental abnormalities.
Conclusions:
- Strong PKCϵ expression in heart and ganglia suggests a significant role in vascular and nervous system development.
- Functional redundancy with other PKC isoforms may explain the lack of defects in PKCϵ-deficient mice.
