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Related Experiment Video

Updated: May 11, 2026

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

Updating the Micro-Tom TILLING platform.

Yoshihiro Okabe¹, Tohru Ariizumi, Hiroshi Ezura

¹Faculty of Life and Environmental Sciences, University of Tsukuba , 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.

Breeding Science

|May 4, 2013

Summary

Researchers have developed a new platform for studying tomato genes using the Micro-Tom variety. This tool, the Micro-Tom Targeting-Induced Local Lesions IN Genomes (TILLING) platform, aids in identifying gene mutations for functional genomics and breeding.

Keywords:

Micro-Tom TILLING reverse genetics tomato

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Area of Science:

Plant Science
Genetics
Molecular Biology

Background:

The dwarf tomato variety Micro-Tom is a key model system for tomato functional genomics.
Existing genomic tools for Micro-Tom include mutant collections and genomic information.
Advances in tomato genome sequencing necessitate accessible reverse genetics tools.

Purpose of the Study:

To develop and describe the Micro-Tom Targeting-Induced Local Lesions IN Genomes (TILLING) platform.
To provide a reverse genetics tool for the tomato research community.
To accelerate the identification of mutant alleles for gene function studies and breeding.

Main Methods:

Development of the Micro-Tom TILLING platform using over 5000 EMS-mutagenized lines.
Utilizing the TILLING approach for efficient mutant allele identification.
Integration with existing Micro-Tom mutant libraries.

Main Results:

The Micro-Tom TILLING platform is established and available.
The platform comprises more than 5000 EMS-mutagenized lines.
It facilitates efficient identification of mutant alleles.

Conclusions:

The Micro-Tom TILLING platform is a valuable reverse genetics tool for tomato research.
This platform, combined with mutant libraries, accelerates mutant isolation.
Ongoing development aims to further enhance Micro-Tom's genomic toolkit.