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Updated: May 29, 2026
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
Published on: April 30, 2016
Development of tomatoes with high sugar and GABA accumulation and parthenocarpic traits by introducing mutations in
Seungje Choi1, Takeru Iwama1, Misaki Kobayashi1
1Graduate School of Life and Earth Sciences, University of Tsukuba, Japan.
Abstract:
Tomatoes are vital crops globally and they provide numerous nutritional benefits, including vitamins, lycopene, and gamma-aminobutyric acid (GABA). This study utilized CRISPR/Cas9 technology to introduce mutations in two genes, HWS (Hawaiian Skirt) and GAD3 (glutamate decarboxylase 3), to enhance tomato quality, increase yield stability, and improve parthenocarpic traits. Our results demonstrated that HWS and GAD3 mutations led to higher sugar (Brix) and GABA content, as well as the induction of facultative parthenocarpy, especially at elevated temperatures. Moreover, facultative parthenocarpy in these mutants was attributed to the defective ability of the transcriptome to be fine-tuned by HWS, which caused more dynamic changes in phytohormone-related fruit development. These findings suggest that genetic modification can improve tomato quality and climate resilience.
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