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Updated: May 11, 2026

Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
Published on: August 5, 2011
[HSP70, an erythropoiesis regulator that determines the fate of erythroblasts between death and differentiation]
O Hermine1, J-B Arlet, J-A Ribeil
1CNRS UMR 8143, labex des globules rouges GR-ex, hôpital Necker, institut Imagine, université Sorbonne Paris Cité, Paris Descartes, Paris, France. ohermine@gmail.com
Abstract:
Erythropoiesis is finely regulated by two major cytokines, stem cell factor (SCF) and erythropoietin (Epo). Decrease levels of Epo result in caspase activation and erythroid progenitors apoptosis. However, normal erythroid cell maturation requests caspase activation and cleavage of various caspase substrates, except the erythroid transcription factor GATA-1, that is protected by interaction with the chaperone HSP70 in the nucleus. Therefore, molecular abnormalities associated with decrease of HSP70 expression in the nucleus may result in ineffective erythropoiesis characterized by apoptosis and impaired maturation of erythroid precursors. These findings open new potential targeted therapies for erythroid disorders.
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