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R gene expression changes related to Cercospora hydrangeae L.
Ioannis Kafantaris1, Pasqualina Woodrow, Petronia Carillo
1Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, Seconda Università di Napoli, via Vivaldi 43, 81100, Caserta, Italy.
Molecular Biology Reports
|May 7, 2013
Summary
Hydrangea leaf spot disease, caused by Cercospora hydrangeae, can be managed by understanding plant resistance. This study identified 20 new resistance gene transcripts in infected Hydrangea macrophylla plants, aiding future disease resistance research.
Area of Science:
- Plant Pathology
- Molecular Biology
- Genomics
Background:
- Hydrangea plants are susceptible to leaf spot fungus Cercospora hydrangeae, which reduces plant vigor and flowering.
- Resistance (R) genes, often encoding nucleotide binding site-leucine rich repeat (NBS-LRR) proteins, are crucial for plant defense against pathogens.
Purpose of the Study:
- To isolate and characterize Hydrangea macrophylla R-gene transcripts.
- To investigate R-gene expression profiles under C. hydrangeae infection.
- To analyze the evolutionary relationships of these R-genes.
Main Methods:
- A cDNA-NBS strategy was employed using cDNAs from control and infected H. macrophylla leaves.
- Phylogenetic analysis was performed to examine evolutionary relationships.
Main Results:
- Twenty new NBS-LRR protein-encoding transcripts were identified exclusively in C. hydrangeae-infected plants.
- Amino acid sequence similarity in the NBS region varied from 45% to 90%, indicating significant diversity among resistance gene analogs (RGAs).
Conclusions:
- The study provides a genomic framework for isolating candidate disease resistance NBS-encoding genes in Hydrangea.
- Findings contribute to understanding the evolutionary dynamics of NBS-encoding genes in Hydrangeaceae crops.
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