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Sema6B, Sema6C, and Sema6D expression and function during mammalian retinal development.

Ryota L Matsuoka¹, Lu O Sun, Kei-ichi Katayama

¹Howard Hughes Medical Institute, The Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.

|May 7, 2013

Summary

Class 6 semaphorins are expressed in the developing retina but do not appear to guide neural circuit formation. These molecules are unlikely to be major ligands for Plexin A receptors in retinal circuit assembly.

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Area of Science:

Neuroscience
Developmental Biology
Molecular Biology

Background:

Neural circuit formation in the vertebrate retina is crucial for visual function.
Repulsive transmembrane semaphorins (Sema6A, Sema5A, Sema5B) and their Plexin A (PlexA) receptors regulate retinal circuit assembly.
Class 6 semaphorins (Sema6B, Sema6C, Sema6D) are known PlexA receptor ligands in other tissues.

Purpose of the Study:

To investigate the roles of Class 6 transmembrane semaphorins (Sema6B, Sema6C, Sema6D) in mammalian retinal development.
To determine if these semaphorins function as PlexA receptor ligands in the retina.

Main Methods:

Expression analysis of Class 6 semaphorins during postnatal retinal development.
Phenotypic analysis of knockout mice (Sema6B-/-, Sema6C-/-, Sema6D-/-) using retinal markers.

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Assessment of neurite stratification in retinal neuron subtypes.

Main Results:

Class 6 semaphorins show specific expression patterns in developing retinal domains and cell types.
No defects in lamina-specific neurite stratification were observed in the knockout retinas.
Sema6B, Sema6C, and Sema6D do not appear to be essential for retinal circuit laminar organization.

Conclusions:

Class 6 transmembrane semaphorins are not major regulators of retinal circuit laminar organization.
These semaphorins likely do not act as key PlexA receptor ligands for this process in the mouse retina.