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Published on: March 21, 2014
Surface modification of electrospun polyvinylidene fluoride nanofiber membrane by plasma treatment for protein
Eugene Cho1, Chan Kim, Ju-Young Park
1Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea.
Abstract:
Electrospun polyvinylidene fluoride (PVDF) nanofiber membranes have 3-dementional (3-D) open pore channel and hence have excellent application potential in Western blot. In this study we have modified electrospun PVDF nanofiber membrane by argon (Ar) plasma treatment to improve the surface hydrophilic and detection sensitivity. The results showed that the detection sensitivity of the Ar plasma-treated PVDF nanofiber membrane increased with increasing plasma treatment time without the need for a methanol pre-wet step. This suggests that the Ar plasma treated PVDF nanofiber membrane can be useful in Western blot with high sensitivity and without methanol pre-wet step.
