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Updated: May 11, 2026

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
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Ramoplanin imaging conjugates--synthesis and evaluation.

Sumbla Sheikh, Alexander Sturzu, Hubert Kalbacher

  • 1Department of Neuroradiology, University of Tübingen, Hoppe-Seyler-Str.3, 72076 Tübingen, Germany. sumblafarooq@yahoo.com.

Medicinal Chemistry (Shariqah (United Arab Emirates))
|May 8, 2013
PubMed
Summary
This summary is machine-generated.

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Ramoplanin imaging conjugates showed weaker uptake and slower cellular penetration in tumor cells compared to vancomycin. This lipoglycodepsipeptide antibiotic induced significant membrane disruption and cell death.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medical Imaging

Background:

  • Previous studies demonstrated vancomycin uptake in human tumor cells.
  • Ramoplanin, a larger lipoglycodepsipeptide antibiotic, offers more coupling sites for imaging agents than vancomycin.

Purpose of the Study:

  • To synthesize and evaluate ramoplanin imaging conjugates for tumor cell uptake and specificity.
  • To compare the cellular uptake and effects of ramoplanin conjugates with vancomycin conjugates.

Main Methods:

  • Synthesis of two fluorescent (FITC, RITC) and one MRI (GdDOTA) ramoplanin conjugates.
  • Evaluation of conjugate uptake, specificity, and accessibility in 8 human cell lines (2 benign, 6 malignant) using CLSM, FACS, and MRI.
  • Assessment of cytotoxicity using propidium iodide and Annexin-V-Fluos assays.

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Last Updated: May 11, 2026

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Main Results:

  • Ramoplanin conjugates exhibited significantly weaker and slower cellular uptake compared to vancomycin conjugates.
  • Uptake of ramoplanin conjugates resulted in substantial cell membrane disruption and induced cell death.
  • Cytoplasmic uptake was observed and quantified via CLSM, FACS, and MRI.

Conclusions:

  • Ramoplanin conjugates are less efficient for tumor cell imaging than vancomycin conjugates due to slower uptake.
  • The observed cytotoxicity of ramoplanin conjugates suggests potential therapeutic applications but limits their use as purely diagnostic imaging agents.