Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate01:25

Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate

The glomerular filtration rate (GFR) is a critical indicator of kidney health, reflecting how well the kidneys filter blood. Changes in GFR can signal potential kidney impairment, necessitating accurate measurement methods to monitor kidney function effectively.Various molecules can serve as markers for GFR measurement, with the ideal marker meeting several specific criteria. It must freely filter at the glomerulus, avoid reabsorption or secretion by the renal tubules, remain unmetabolized, not...
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area. This equation is...
Renal Drug Excretion: Glomerular Filtration01:02

Renal Drug Excretion: Glomerular Filtration

The kidney serves as the primary organ responsible for eliminating drugs and their metabolites from the body. This process, known as renal elimination, starts with glomerular filtration and results in urine formation. Each kidney houses millions of functional units called nephrons, where urine production occurs. A nephron has two main components: a renal corpuscle and a renal tubule.
Drugs gain access to the kidney via the renal artery, which progressively branches off into afferent arterioles.
Renal Clearance01:23

Renal Clearance

The glomerular filtration rate (GFR) is a critical marker of kidney function, reflecting the efficiency of filtration by the glomeruli. Renal clearance of specific substances, such as inulin or creatinine, is commonly used to measure GFR.
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...
Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance01:25

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance

In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance (Clcr), a...
One-Compartment Open Model: Urinary Excretion Data and Determination of k01:11

One-Compartment Open Model: Urinary Excretion Data and Determination of k

The one-compartment open model leverages urinary excretion data to estimate renal clearance, which gauges the kidney's capacity to expel a drug. This method offers several benefits, including directly measuring drug elimination and assessing the kidney's contribution to overall drug clearance. However, this approach has limitations. It assumes sole renal excretion of the drug, which is not true for all drugs. Accurate urinary excretion and plasma drug concentration measurement can also be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urinary extracellular vesicles as a source of protein-based biomarkers in feline chronic kidney disease and hypertension.

The Journal of small animal practice·2022
Same author

First genome-wide association study investigating blood pressure and renal traits in domestic cats.

Scientific reports·2022
Same author

Investigation of the transforming growth factor-beta 1 signalling pathway as a possible link between hyperphosphataemia and renal fibrosis in feline chronic kidney disease.

Veterinary journal (London, England : 1997)·2020
Same author

Characterisation of Crandell-Rees Feline Kidney (CRFK) cells as mesenchymal in phenotype.

Research in veterinary science·2019
Same author

Associations between single nucleotide polymorphisms in the calcium sensing receptor and chronic kidney disease-mineral and bone disorder in cats.

Veterinary journal (London, England : 1997)·2018
Same author

Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor β1.

BMC veterinary research·2018
Same journal

Dietary-responsive disseminated eosinophilia in a border terrier.

Journal of veterinary internal medicine·2026
Same journal

Clinical features, diagnostic findings, and outcomes of emphysematous cholecystitis in 35 dogs: a retrospective case series.

Journal of veterinary internal medicine·2026
Same journal

Characterization and outcomes of cats with cardiomyopathy undergoing subcutaneous ureteral bypass device placement.

Journal of veterinary internal medicine·2026
Same journal

Multisite sampling improves consensus cytologic interpretation in bone marrow evaluation of dogs.

Journal of veterinary internal medicine·2026
Same journal

Efficacy and safety of canagliflozin in insulin dysregulated horses: a 4-week multi-arm, double-blind, randomized, clinical trial.

Journal of veterinary internal medicine·2026
Same journal

Use of C-peptide and homeostasis model assessment of insulin resistance to assess insulin resistance in dogs.

Journal of veterinary internal medicine·2026
See all related articles

Related Experiment Video

Updated: May 11, 2026

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
07:07

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

A single sample method for estimating glomerular filtration rate in cats.

N C Finch1, R Heiene, J Elliott

  • 1Royal Veterinary College, London, UK. natalie.finch@bristol.ac.uk

Journal of Veterinary Internal Medicine
|May 8, 2013
PubMed
Summary
This summary is machine-generated.

A new modified Jacobsson method accurately estimates glomerular filtration rate (GFR) in cats using a single blood sample. This method, utilizing a 180-minute sample and a body weight-based formula, simplifies GFR assessment in feline patients.

Keywords:
ClearanceFunctionIohexolRenal

More Related Videos

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat
06:58

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat

Published on: July 26, 2015

Transdermal Measurement of Glomerular Filtration Rate in Mice
07:25

Transdermal Measurement of Glomerular Filtration Rate in Mice

Published on: October 21, 2018

Related Experiment Videos

Last Updated: May 11, 2026

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice
07:07

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

Published on: May 10, 2013

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat
06:58

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat

Published on: July 26, 2015

Transdermal Measurement of Glomerular Filtration Rate in Mice
07:25

Transdermal Measurement of Glomerular Filtration Rate in Mice

Published on: October 21, 2018

Area of Science:

  • Veterinary Medicine
  • Nephrology
  • Diagnostic Imaging

Background:

  • Accurate estimation of glomerular filtration rate (GFR) is crucial for feline health management.
  • Current validated methods for GFR assessment in cats often require multiple blood samples, posing practical challenges.

Purpose of the Study:

  • To evaluate a novel single-sample method for determining GFR in cats.
  • To validate the accuracy of the modified Jacobsson method for GFR estimation.

Main Methods:

  • Iohexol clearance was used to determine GFR in a validation group (n=89) and a testing group (n=18) of cats.
  • Single sample clearance was assessed using Jacobsson and modified Jacobsson methods, with extracellular fluid volume (ECFV) predicted from body weight.
  • The optimal single sample method was identified and tested in a separate cohort.

Main Results:

  • The modified Jacobsson method, using a blood sample at 180 minutes and a predicted ECFV, demonstrated agreement with multisample clearance in healthy cats.
  • The Jacobsson method tended to overestimate multisample clearance, while the modified Jacobsson method showed comparable results (2.22 ± 0.34 mL/min/kg vs. 2.19 ± 0.34 mL/min/kg).

Conclusions:

  • The modified Jacobsson method offers a reliable and simplified approach for estimating GFR in cats from a single blood sample.
  • This method, incorporating a body weight-based ECFV prediction, has significant potential for routine clinical use.
  • Further studies are recommended to validate this method in cats with extreme GFR values.